Arabidopsis Qc-SNARE genes BET11 and BET12 are required for fertility and pollen tube elongation

Botanical Studies

Table 1 Transmission of the heterozygous bet11-1 and bet12-1 alleles is impaired in bet11/12 double mutants

Cross	Segregating T-DNA allele	Expected segregation rate (E)	Observed segregation rate (O)	Transmission efficiency (O/E) × 100 (%)
bet11 HM/bet12 HZ (female) × WT (male)	bet12	50	21	42
WT (female) × bet11 HM/bet12 HZ	bet12	50	11	22
bet12 HM/bet11 HZ (female) × WT (male)	bet11	50	12	24
WT (female) × bet12 HM/bet11 HZ	bet11	50	7	14

Results show the segregation rates of the bet11-1 and bet12-1 alleles after reciprocal crosses involving the wild type (WT) and bet11/bet12 double mutants in either the homozygous/heterozygous (HM/HZ) or the HZ/HM condition. From left to right, the table indicates the expected Mendelian segregation rate (E), the actual observed segregation rate (O) and the transmission efficiency (O/E × 100). For the bet11 HM/bet12 HZ line, maternal transmission efficiency of the bet12-1 heterozygous (HZ) allele was low (42 %). Even lower values in the transmission efficiency of bet12-1 allele were observed when the T-DNA allele was transmitted paternally through pollen (22 %). For the bet11 HZ/bet12 HM line, maternal and paternal transmission efficiencies of the heterozygous bet11-1 allele were low (24 and 14 %). Data was collected from 100 segregating seedlings per cross