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Botanical Studies
Fig. 6 | Botanical Studies

Fig. 6

From: Two conserved oligosaccharyltransferase catalytic subunits required for N-glycosylation exist in Spartina alterniflora

Fig. 6

Phenotypic complementation of the SaSTT3A on the Arabidopsis mutant. a The phenotypic observation of transgenic seedlings grown on 150 mM NaCl stress medium. b The statistic analysis of the primary root length of seedlings expressing 35S:SaSTT3. c The statistic analysis of the primary root length of seedlings expressing AtSTT3A:SaSTT3. d Immunoblot analysis of PDI. Total protein extracted from seedlings of WT, stt3a-2, 35S:SaSTT3A/stt3a-2, 35S:SaSTT3B/stt3a-2 were separated by SDS-PAGE and analyzed by immunoblotting with anti-PDI antibody. The PDI band and underglycosylated protein form were examined and pointed by the arrows, respectively. Coommassie bright blue stained Rubisco small subunit was used as the loading control

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