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Botanical Studies

Table 1 Purification of enzymes catalyzing the p-nitrophenyl butyrate reaction from Pichia pastoris

From: Arabidopsis SFAR4 is a novel GDSL-type esterase involved in fatty acid degradation and glucose tolerance

Purified step

Total protein (mg)

Total activity (μM min−1)

Yield (%)

Specific activity (μM min−1 mg−1 protein)

Enrichment (fold)

Secreted medium (20 times concentrate)

2.00

7.16

100

3.58

1

HisTrap Sepharose

0.15

0.94

13.10

6.25

1.75

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