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Table 1 Purification of enzymes catalyzing the p-nitrophenyl butyrate reaction from Pichia pastoris

From: Arabidopsis SFAR4 is a novel GDSL-type esterase involved in fatty acid degradation and glucose tolerance

Purified step Total protein (mg) Total activity (μM min−1) Yield (%) Specific activity (μM min−1 mg−1 protein) Enrichment (fold)
Secreted medium (20 times concentrate) 2.00 7.16 100 3.58 1
HisTrap Sepharose 0.15 0.94 13.10 6.25 1.75