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Table 2 Influence of different light spectra on induction of somatic embryos and callus growth in P. japonicum

From: Influence of LED light spectra on in vitro somatic embryogenesis and LC–MS analysis of chlorogenic acid and rutin in Peucedanum japonicum Thunb.: a medicinal herb

Light spectrum Total number of somatic embryos Percentage of vitrified somatic embryos Average callus fresh weight (mg/clump)a Callus texture
CW-5000 K 110 (g) 20 732 ± 37 b Compact, dry, pink color
WW-2700 K 90 (g + t) 31 644 ± 14 de Compact, succulent
7R1G1B 191 (g + t) 20 635 ± 18 def Compact, succulent
8R1B 329 (g + t) 20 555 ± 14 ef Compact, light golden color
3R3B3IR 0 0 721 ± 16 bc Compact, dry, with white cells
9IR 0 0 821 ± 15 a Compact, deep golden color
9R 0 0 631 ± 6 def Compact, light creamish color, succulent
9B 0 0 681 ± 11 bcd New callus friable, purple color, succulent
  1. Culture medium: MS basal medium + 3 % sucrose + 0.4 % GPP. Total number of callus clumps each petridish = 20; each callus clump = 100 mg; observations at 60 days of culture
  2. g globular, t torpedo
  3. aMean ± standard error. Means within each column followed by the same letter(s) are not significantly different at 5 % level by Fisher's protected LSD test