Influence of LED light spectra on in vitro somatic embryogenesis and LC–MS analysis of chlorogenic acid and rutin in Peucedanum japonicum Thunb.: a medicinal herb

Botanical Studies

Table 2 Influence of different light spectra on induction of somatic embryos and callus growth in P. japonicum

Light spectrum	Total number of somatic embryos	Percentage of vitrified somatic embryos	Average callus fresh weight (mg/clump)^a	Callus texture
CW-5000 K	110 (g)	20	732 ± 37 b	Compact, dry, pink color
WW-2700 K	90 (g + t)	31	644 ± 14 de	Compact, succulent
7R1G1B	191 (g + t)	20	635 ± 18 def	Compact, succulent
8R1B	329 (g + t)	20	555 ± 14 ef	Compact, light golden color
3R3B3IR	0	0	721 ± 16 bc	Compact, dry, with white cells
9IR	0	0	821 ± 15 a	Compact, deep golden color
9R	0	0	631 ± 6 def	Compact, light creamish color, succulent
9B	0	0	681 ± 11 bcd	New callus friable, purple color, succulent

Culture medium: MS basal medium + 3 % sucrose + 0.4 % GPP. Total number of callus clumps each petridish = 20; each callus clump = 100 mg; observations at 60 days of culture
g globular, t torpedo
^aMean ± standard error. Means within each column followed by the same letter(s) are not significantly different at 5 % level by Fisher's protected LSD test