Fig. 1

Purification and bioactive analyses of Hpa1 and Hpa1_10–42 proteins in comparison with the control protein EVP. a Characterization of proteins by electrophoresis. The Hpa1-His and Hpa1_10–42-His fusion proteins were produced after genetic combination in a prokaryotic expression vector. The empty vector preparation (EVP) was produced by using the vector without genetic combination. Proteins before and after purification were analyzed by electrophoresis in dodecyl sulphate-plolyacrylamide gel. Protein bands were visualized by gel staining with Coomassie Brilliant Blue G-250. b The bioactivity assay of proteins based on the induction of hypersensitive response (HR) in tobacco leaves. The his tag had been removed before the assay; aqueous solution of proteins were infiltrated into intercellular spaces of the leaves; leaves were photographed on 2 days after infiltration; and the HR appeared as local necrosis