Fig. 8

Nile red staining fluorescence micrograph of developing embryos of G. nantoensis. a Nile red staining fluorescence micrograph of a late globular embryo at the same stage as that seen in Fig. 7g. The innermost walls of the seed coat (arrows) and the surface wall (arrowhead) of the embryo proper react positively to the stain. The fluorescence is absent in the suspensor wall (double arrowhead). Scale bar 50 μm. b Nile red staining fluorescence micrograph of a mature seed at the same stage as that seen in Fig. 7h. The seed coat (arrow) and the surface wall (arrowhead) of the embryo proper react positively to the stain. Scale bar 50 μm