Accumulation of catechins and expression of catechin synthetic genes in Camellia sinensis at different developmental stages

Botanical Studies

Table 1 Primers of catechin biosynthetic genes for real-time PCR analysis in Camellia sinensis

Gene	Accession no.	Primer sequence 5′−3′
CHS1	D26593	Forward: CAGAGCACGTACCCGGATTATTAC
CHS1	D26593	Reverse: GGTACTTCAACCACAACCATGTCC
CHS2	D26594	Forward: CATTACTAACAGCGAGCATAAAACG
CHS2	D26594	Reverse: TAGTTTTGGGACTTCAACAACCAC
CHS3	D26595	Forward: CCTCTTCCTAGCTAGCACATACCA
CHS3	D26595	Reverse: TGCTCGCTATTAGTAATCCGAAAGT
ANS	GAAC01051116	Forward: TGGGAAGACTATTTCTTCCACCTT
ANS	GAAC01051116	Reverse: TTCTAGTCGGCCTTCTTCTAGTCC
ANR1	GU944768	Forward: CCTTCTAGCACTAAAGGGTTCAGG
ANR1	GU944768	Reverse: TGACTACTCCTTGAATTGCTGGTT
ANR2	JN024667	Forward: TCACAGGGAATGAATTCCTCATAG
ANR2	JN024667	Reverse: GGAACATTGTACTGGGGGTATCTT
LAR	EF205148	Forward: TTTTGTTGCAGGCTCTGATATAGG
LAR	EF205148	Reverse: ATTATATTCACTGCTGCTGCTGCT
18S rRNA	AB120309.1	Forward: TCTCAACCATAAACGATGCCGACCAG
18S rRNA	AB120309.1	Reverse: TTTCAGCCTTGCGACCATACTCCC