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Fig. 2 | Botanical Studies

Fig. 2

From: Efficient and heritable transformation of Phalaenopsis orchids

Fig. 2

Confirmation of Phalaenopsis transgenic lines. a Construction map of Ubi:eGFP used in this study. Schematic structure of the T-DNA region in the vector of pZp200. CaMV 35S, promoter of CaMV 35S; HptII, coding region of hygromycin phosphotransferase gene; eGFP, coding region of eGFP; NosT, terminator of nopaline synthase gene; LB left border; RB right border. b Genomic PCR analysis of eGFP and HptII genes. −CT, negative control without addition of the genomic DNA template; +CT, with added plasmid DNA. c Southern blot analysis of HptII showing integration of T-DNA in Phalaenopsis orchid genome. Transgenic lines and their respective Wt plants were analyzed side by side

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