Fig. 5
From: Efficient and heritable transformation of Phalaenopsis orchids
Stable inheritance of transgene in BC1 orchid progeny. a Pollinia of D1 transgenic line was crossed-pollinated to four distinct genetic backgrounds of Wt orchids and generated BC1 progenies. Ama (4n)/D1 BC1 seeds were sown on 1/2 MS agar plates containing 25 ppm hygromycin. Some BC1 progenies were resistant to hygromycin and some were susceptible showing white and wilt symptoms. Red arrows indicate hygromycin susceptible explants. b Record of survival rate in BC1 transgenic lines. c Genomic PCR showed HptII gene integration in the hygromycin-resistant BC1 progenies. d Western blot showed GFP protein expression in the hygromycin-resistant BC1 progenies. Ama (4n) was used as the non-transgenic wild-type negative control. D1, the original T0 transgenic parent was used as a positive control