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Fig. 4 | Botanical Studies

Fig. 4

From: Antioxidants, anti-inflammatory, and antidiabetic effects of the aqueous extracts from Glycine species and its bioactive compounds

Fig. 4

Inhibition of iNOS and COX-2 protein expression by genistein and daidzein in LPS-stimulated RAW264.7 cells. Cells were incubated for 24 h with 100 μg/mL of LPS in the absence or the presence of genistein or daidzein (5 and 10 μg/mL). Samples were added 1 h before incubation with LPS. Lysed cells were then prepared and subjected to Western Blotting using an antibody specific for iNOS and COX-2 (a). The values under each lane indicate the relative band intensities normalized to β-actin (b). β-actin was used as a quantity control. The data were presented as mean ± SD for three different experiments performed in triplicate

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