Fig. 1

Representative results of seedling pathogenicity assays, SCAR types, and PCR–RFLP diagnosis. a The pathogenicity assay with seedling coleoptile inoculation method was used to test if all isolates are pathogenic to wheat. Necrotic leaf sheath lesions (arrows) indicated that the tested isolates were FHB pathogens. No symptom displayed after inoculation with sterilized water or Fusarium oxysporum f.sp. lycopersici (Fol, not a FHB pathogen), respectively. b The variation of SCAR types in studied population. The first lane is the DNA ladder; Lane 1 is SCAR type 1 (410 bp); Lane 2 is SCAR type 2 or type 5 (497 bp); Lane 3 is SCAR type 3 (527 bp); Lane 4 is SCAR type 4 (557 bp). The last lane is another DNA ladder. c A PCR–RFLP method for diagnosis of F. asiaticum and F. graminearum s. str. through a partial histone H3 fragment. A F. asiaticum isolate will be digested by StyI but not EcoRV whereas a F. graminearum s. str. isolate will be digested by EcoRV but not StyI. Other species will not be digested by the two enzymes. The first lane is the DNA ladder; Lanes 1 to 3 are a F. asiaticum isolate (Pingzhen1-49); Lanes 4 and 5 are a F. graminearum s. str. isolate (Daya211-13); Lanes 6 and 7 are an unknown species isolate (Daya272-3). Different restriction enzyme treatments are indicated: for “S” this is StyI, for “E” this is EcoRV, and for “U” this is the uncut control