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Table 2 The leaf anatomical characters and character states used in phenetic analyses

From: Identification among morphologically similar Argyreia (Convolvulaceae) based on leaf anatomy and phenetic analyses

Anatomical characters Character states Score
Qualitative characters
 1. Epidermal cell wall on adaxial leaf surface Straight to slightly curved/sinuate 0/1
 2. Epidermal cell wall on abaxial leaf surface Straight to slightly curved/sinuate 0/1
 3. Types of non-glandular trichome on abaxial leaf surface Large and small straight trichome/large straight and small curly trichome 0/1
 4. Shape of petiole Slightly concave/convex 0/1
 5. Midrib outline and adaxial curvature Rounded/acute 0/1
 6. Leaf margin shape Rounded/acute 0/1
Quantitative characters
 7. Ratio of adaxial leaf epidermal cell size (width: length) Ratio is less than or equal to 0.7/ratio is more than 0.7 0/1
 8. Stomatal index (SI) on adaxial leaf surface SI is less than or equal to 6/SI is more than 6 0/1
 9. Ratio of stomatal size on adaxial leaf surface (width: length) Ratio is less than or equal to 0.6/ratio is more than 0.6 0/1
 10. Ratio of glandular trichome size on adaxial leaf surface (width: length) Ratio is less than or equal to 0.92/ratio is more than 0.92 0/1
 11. Ratio of non-glandular trichome size on adaxial leaf surface (width: length) Ratio is less than or equal to 0.08/ratio is more than 0.08 0/1
 12. Ratio of abaxial leaf epidermal cell size (width: length) Ratio is less than or equal to 0.65/ratio is more than 0.65 0/1
 13. Stomatal index (SI) on abaxial leaf surface SI is less than or equal to 15/SI is more than 15, but less than or equal to 30/SI is more than 30 0/1/2
 14. Ratio of stomatal size on abaxial leaf surface (width: length) Ratio is less than or equal to 0.75/ratio is more than 0.75 0/1
 15. Ratio of glandular trichome size on abaxial leaf surface (width: length) Ratio is less than or equal to 0.9/ratio is more than 0.9 0/1
 16. Ratio of non-glandular trichome size on abaxial leaf surface (width: length) Ratio is less than or equal to 0.03/ratio is more than 0.03, but less than or equal to 0.04/ratio is more than 0.04 0/1/2
 17. Ratio of epidermal cells size in petiole (width: length) Ratio is less than or equal to 0.7/ratio is more than 0.7 0/1
 18. Ratio of petiole transverse section (width: length) Ratio is less than or equal to 0.82/ratio is more than 0.82, but less than or equal to 0.95/ratio is more than 0.95 0/1/2
 19. Ratio of palisade mesophyll cell size in leaf transverse section (width: length) Ratio is less than or equal to 0.2/ratio is more than 0.2 0/1
 20. Ratio of spongy mesophyll cell size in leaf transverse section (width: length) Ratio is less than or equal to 0.7/ratio is more than 0.7 0/1
 21. Ratio of adaxial epidermal cell size in leaf transverse section (width: length) Ratio is less than or equal to 0.7/ratio is more than 0.7 0/1
 22. Ratio of abaxial epidermal cell size in leaf transverse section (width: length) Ratio is less than or equal to 0.7/ratio is more than 0.7 0/1
 23. Blade thickness (µm) Thickness is less than or equal to 208/thickness is more than 208, but less than or equal to 248/thickness is more than 248 0/1/2
 24. Ratio of midrib thickness (width: length) Ratio is less than or equal to 1/ratio is more than 1 0/1