Table 1 Primer sequence for starch synthesis related gene
From: Starch accumulation in hulless barley during grain filling
Gene | NCBI accession | Forward primers (5′–3′) | Reverse primers (3′–5′) | Annealing temperature (°C) |
|---|---|---|---|---|
AGPP | AK359692 | TTCAGTTTCATGACCGTTC | AGTATCCATCTGTCTCCCTC | 60 |
GBSS I | AY050174 | GACACTATCGTGGAAGGCAAG | TTGACCATCTCATGGTACGC | 60 |
GBSS II | AF109395 | CACAGAATGCCAGAGGCATAG | GAACAGATGGGAATCACTCCA | 60 |
SSS I | AJ292521 | GCAAAAGGAGAGGAGGGTACA | ACGTATGGTCTTTCGTCATGC | 60 |
SSS II | AB201445 | GCTACACCAACTTCTCCCTG | GATGATCTCCACGCCCTTCT | 60 |
SSS III | AF258608 | GACATGTGGTTTTGCTTGGTT | AGCCAGCGTATATCAGGTGAG | 60 |
SBE I | AF286317 | ATGTTTGGTGGACATGGAAGA | ATGTTTGGTGGACATGGAAGA | 60 |
SBE IIa | Y11282 | ATGTTTGGTGGACATGGAAGA | ATGTTTGGTGGACATGGAAGA | 60 |
SBE IIb | AY740401 | ATGTTTGGTGGACATGGAAGA | ATGTTTGGTGGACATGGAAGA | 60 |
Actin | DN551593 | GGAAAAGTGCAGAGAGACACG | TACAGTGTCTGGATCGGTGGT | 60 |