Transcriptomic analyses reveal clathrin-mediated endocytosis involved in symbiotic seed germination of Gastrodia elata

Botanical Studies

Table 2 The up- and down-regulated genes in protocorms of G. elata compared with seeds

Unigene ID	Putative gene	Refseq	Ratio change
c113523_g1	CHC	XP_010907410.1	2.56*
c113909_g1	CLC	XP_008802347.1	1.89
c29883_g1	AP-1 β	XP_009385856.1	1.92
c36857_g1	AP-1 μ	XP_010923038.1	1.28
c49102_g1	AP-1 σ	AFS50008.1	2.26*
c98691_g1	AP-1 γ	XP_010913560.1	2.31*
c29046_g1	AP-2 μ	XP_007039526.1	1.68
c33125_g1	AP-2 α	XP_010933729.1	2.59*
c126705_g1	AP-3 δ	XP_009396369.1	0.9
c29490_g1	AP-3 σ	XP_010939294.1	1.54
c46833_g1	AP-3 β	XP_010937611.1	0.95
c50113_g1	AP-3 μ	XP_010921397.1	1.17
c43068_g1	AP-4 ε	XP_010909540.1	2.09*
c50920_g1	AP-4 σ	XP_007152949.1	2.39*
c28875_g1	Adaptin	XP_008792344.1	2.40*
c50842_g1	AP180	XP_009396332.1	2.18*
c36930_g2	Epsin 1	XP_008799757.1	2.04*
c50396_g1	Epsin 2	XP_009392171.1	1.62
c45022_g1	Epsin 3	XP_010922341.1	0.96
c29413_g1	DRP3	XP_008794571.1	3.07*
c32547_g1	DRP5	XP_010918171.1	3.84*
c43852_g2	DRP1	XP_010933528.1	5.75*
c56183_g1	DRP2	XP_008779526.1	2.48*
c29657_g1	HSC70	XP_011094842.1	2.08*
c33306_g1	HSC70	XM_009382388.1	2.45*
c7920_g1	HSC70	XP_011027182.1	2.23*

Only differential expression genes with change ratios ≥2 (up) or ≤0.5 (down) were marked with asterisk (*). Transcriptomic analyses were performed on the seeds and protocorms of G. elata. Of the 26 genes involved in clathrin-mediated endocytosis, 16 genes were found to be significantly up-regulated in the protocorms based on RNA-seq analyses. CHC clathrin heavy chain, CLC clathrin light chain, AP-1 β/μ/σ/γ adaptor protein-1 complex subunit beta/mu/sigma/gamma, AP-2 α/μ adaptor protein-2 complex subunit alpha/mu, AP-3 δ/σ/β/μ adaptor protein-3 complex subunit delta/sigma/beta/mu, AP-4 ε/σ adaptor protein-4 complex subunit epsilon/sigma, AP180 clathrin assembly protein, espin clathrin interactor epsin, DRP dynamin-related protein, HSC70 heat shock cognate 70 kDa