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Table 1 List of taxa from Itremo, Central Highlands, Madagascar: seed capsule size and seed capsule colour 2–3 weeks after in vitro collecting and results of seed capsule sterilisation that yielded clean seeds 3 weeks after sowing

From: Collecting near mature and immature orchid seeds for ex situ conservation: ‘in vitro collecting’ as a case study

Taxon Life form Colour of seed pod on arrival at RBG Kew Size group Init ial pod sterilisat ion t ime (mins) Response at step 1, 2 and 3 [Sterile (S)/Contaminated (C)] Response after seed sowing
1 2 1
Aerangis citrata EP Green 4 45 S S
Aerangis ellisii LP Green + brown 2 80 C S S
Aerangis ellisii LP Green, majority brown 2 80 C S C
Aerangis punctata EP Reddish tinge 4 60 S S
Aerangis punctata EP Brown 4 50 C S S
Aerangis sp. LP Green 4 40 S S  
Aerangis sp. LP Green 3 40 S S  
Aerangis sp. LP Green 3 40 S S  
Aerangis sp. LP Yellowish brown 3 60 C C S S
Angraecum calceolus LP Green 3 50 S S
Angraecum magdalenae LP Green/brown 1 90 C S S
Aerangis protensum LP, TR Greenish/yellow brownish 2 60 C S S
Aerangis rutenbergianum LP Brown 3 60 C S S
Aerangis rutenbergianum LP Green 4 50 S S
Aerangis sororium LP Green 1 90 S S
Aerangis sororium LP Brown 1 90 C S S
Aerangis sp. LP Greenish brown 5 40 S S
Bulbophyllum peyrotii EP Brownish black 5 60 C S S
Habenaria ambositrana TR Green 5 60 S S
Oeceoclades calcarata LP Brown 1 80 C S S
Polystachya fusiformis LP, EP Green 4 45 C S S
Polystachya fusiformis LP, TR Green/brown 5 60 S S
  1. Capsule size group: above 1500 mm2 (1), above 750 mm2 (2), above 300 mm2 (3), above 150 mm2 (4), below 150 mm2 (5)
  2. TR terrestrial, EP epiphyte, LP lithophyte as found in the field. Response: C contaminated, S sterile