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Table 1 List of taxa from Itremo, Central Highlands, Madagascar: seed capsule size and seed capsule colour 2–3 weeks after in vitro collecting and results of seed capsule sterilisation that yielded clean seeds 3 weeks after sowing

From: Collecting near mature and immature orchid seeds for ex situ conservation: ‘in vitro collecting’ as a case study

Taxon

Life form

Colour of seed pod on arrival at RBG Kew

Size group

Init ial pod sterilisat ion t ime (mins)

Response at step 1, 2 and 3 [Sterile (S)/Contaminated (C)]

Response after seed sowing

1

2

1

Aerangis citrata

EP

Green

4

45

S

S

Aerangis ellisii

LP

Green + brown

2

80

C

S

S

Aerangis ellisii

LP

Green, majority brown

2

80

C

S

C

Aerangis punctata

EP

Reddish tinge

4

60

S

S

Aerangis punctata

EP

Brown

4

50

C

S

S

Aerangis sp.

LP

Green

4

40

S

S

 

Aerangis sp.

LP

Green

3

40

S

S

 

Aerangis sp.

LP

Green

3

40

S

S

 

Aerangis sp.

LP

Yellowish brown

3

60

C

C

S

S

Angraecum calceolus

LP

Green

3

50

S

S

Angraecum magdalenae

LP

Green/brown

1

90

C

S

S

Aerangis protensum

LP, TR

Greenish/yellow brownish

2

60

C

S

S

Aerangis rutenbergianum

LP

Brown

3

60

C

S

S

Aerangis rutenbergianum

LP

Green

4

50

S

S

Aerangis sororium

LP

Green

1

90

S

S

Aerangis sororium

LP

Brown

1

90

C

S

S

Aerangis sp.

LP

Greenish brown

5

40

S

S

Bulbophyllum peyrotii

EP

Brownish black

5

60

C

S

S

Habenaria ambositrana

TR

Green

5

60

S

S

Oeceoclades calcarata

LP

Brown

1

80

C

S

S

Polystachya fusiformis

LP, EP

Green

4

45

C

S

S

Polystachya fusiformis

LP, TR

Green/brown

5

60

S

S

  1. Capsule size group: above 1500 mm2 (1), above 750 mm2 (2), above 300 mm2 (3), above 150 mm2 (4), below 150 mm2 (5)
  2. TR terrestrial, EP epiphyte, LP lithophyte as found in the field. Response: C contaminated, S sterile