Identification of seeds based on molecular markers and secondary metabolites in Senna obtusifolia and Senna occidentalis

Botanical Studies

Table 2 Genetic polymorphism parameters of 36 samples generated by 3 ISSR and 7 SCoT primers

Primer	Primer sequence	Total bands		Polymorphic bands		PPB (%)
Primer	(5′→3′)	Sob	Soc	Sob	Soc	Sob	Soc
UBC880	(GA)8YG	29	21	20	15	68.9	71.4
UBC881	GGGT(GGGGT)2G	23	17	17	12	73.9	70.6
UBC885	BDB(CA) 7	18	16	14	8	77.8	50.0
Average	–	23.3	18.0	17.0	11.7	73.5	64.0
Total	–	70	54	51	35	72.9	64.8
SCoT 8	ACCATGGCTACCACCGCA	20	15	18	12	90.0	80.0
SCoT 21	CCATGGCTACCACCGCCT	24	17	20	14	83.3	82.4
SCoT 27	ACAATGGCTACCACTGCC	16	11	15	10	93.8	90.9
SCoT 42	ACCATGGCTACCACCGGC	18	11	14	10	77.8	90.9
SCoT 43	ACCATGGCTACCACCGCC	15	12	11	11	73.3	91.7
SCoT 44	ACGACATGGCGACCCACA	17	13	16	11	94.1	84.6
SCoT 66	ACAATGGCTACCACTAGC	21	13	18	10	85.7	76.9
Average	–	18.7	13.1	16.0	11.1	85.4	85.7
Total	–	131	92	112	78	85.5	84.8

Sob: S. obtusifolia; Soc: S. occidentalis; PPB: percentage of polymorphic bands