Table 2 Characteristics of the 28 polymorphic microsatellite primers derived from Phalaenopsis aphrodite subsp. formosana
From: Screening transferable microsatellite markers across genus Phalaenopsis (Orchidaceae)
Locus | Primer sequences (5′–3′) | Repeat motif | Allelic size (bp) | Annealing temperature (°C) | No. of alleles | PIC |
|---|---|---|---|---|---|---|
PA5-1 | F: TCCCATTATCACTCCCTCAC | (TC)14 | 140–164 | 59 | 4 | 0.67 |
R: GGTTAGAGATATAGGGAGAG | ||||||
PA5-2 | F: CTCTCTTTCCTTCTCACCTC | (TC)10 | 98–104 | 58 | 4 | 0.61 |
R: AAGATAGAGGGAGAGAGTGG | ||||||
PA7 | F: CTCTGCTTCTCACCTTTCAC | (TC)12 | 116–264 | 56 | 3 | 0.55 |
R: GGACAGAAAGTGAGAGAGAG | ||||||
PA10 | F: TCTTCAGTCCCTCACTCATC | (CT)14 | 132–152 | 58 | 7 | 0.75 |
R: ACAAAGCGGTGGAGAATATG | ||||||
PA11 | F: ATCTATTGCTCTTTGTCCTC | (CT)42 | 214–216 | 55 | 2 | 0.38 |
R: TAGCAAAGAGATGCTGAAGG | ||||||
PA14 | F: TTTTCACTCTCCCTCCATCC | (CT)21 | 182–186 | 52 | 3 | 0.55 |
R: GATGTAGAGAATGAGGGAGC | ||||||
PA15 | F: TCTCCTACTCCCTCTATCTCA | (CT)25 | 306–310 | 56 | 3 | 0.55 |
R: CTTGAAAGGCAGAGAGATAG | ||||||
PA19 | F: TCTCCCTATATCTCTGCATC | (CTCC)4 | 154–158 | 51 | 3 | 0.58 |
R: TGGAAAGAGAAAGGTTCAGG | ||||||
PA21 | F: TCTCTCACTTTGTCACTCGC | (CT)14 | 134–146 | 57 | 6 | 0.77 |
R: AAAGGGAAGTAGGGAAGGAG | ||||||
PA24 | F: TTGATCTCTCTGGCACCCAC | (TC)36 | 216–224 | 55 | 3 | 0.59 |
R: AAGAGAGAGTTAGTTGGAGAT | ||||||
PA25-1 | F: ACCCACTTTCTCCTATCTCC | (CT)20 | 176–202 | 58 | 5 | 0.64 |
R: GATGAAAGAGAGTGAGAGCG | ||||||
PA25-2 | F: TCTCCCTCTCTTTACCACTC | (CT)12 | 92–267 | 58 | 6 | 0.73 |
R: GTGAGAGAGATAGAGTGAGC | ||||||
PA32-1 | F: CTCTTCCTGCTTTTCCTAGG | (CT)25 | 148–222 | 57 | 8 | 0.83 |
R: AAGAGGGTGTGAGGAAGAGG | ||||||
PA32-2 | F: TCTCTCACTACTCTATCTTG | (CT)18 | 140–152 | 54 | 5 | 0.73 |
R: GAGAAGATAGAAAGAGTGAG | ||||||
PA36 | F: CTCCACTTTATCTCTCTACC | (TC)39 | 220–250 | 55 | 4 | 0.67 |
R: ATTGAGCGAGATAAAACTAG | ||||||
PA37 | F: TTTACCTCTTTTGCTAGCTC | (TC)23 | 226–234 | 50 | 4 | 0.67 |
R: AAGAGAAAGGGAAGGAGAGC | ||||||
PA38 | F: CTCTCTCACTCTATTACTCC | (CT)32 | 224–384 | 54 | 9 | 0.84 |
R: AGCTAGATAGAGGGAGAAAG | ||||||
PA40 | F: GAGCAACATTCACTAGAGAG | (CA)14 | 258–320 | 56 | 6 | 0.79 |
R: CTGGCAAAGCTTTGAGAAGG | ||||||
PA41 | F: GAGGAGAAATAATGATTCCG | (AG)12 | 138–140 | 50 | 2 | 0.38 |
R: AGACACTCTCACACACTTTC | ||||||
PA63 | F: TTCATTCCATCTACCCCATC | (CT)8 | 130–136 | 55 | 3 | 0.59 |
R: GATAGAAAGACTAGAGTAGG | ||||||
PA64 | F: CTCTCCTTTTTCTTATCTTTCAC | (CT)94 | 248–296 | 55 | 3 | 0.55 |
R: TAGAGAGATAGAGGGCAAGC | ||||||
PA74-1 | F: AATGACCTCTCTGCTCTCTC | (TC)28 | 172–306 | 50 | 3 | 0.59 |
R: GCAAGAGAAGTTGTGGGATGG | ||||||
PA74-2 | F: CATCCCACAACTTCTCTTGC | (CT)13 | 122–134 | 55 | 5 | 0.58 |
R: AGTGCTCAAGCGAGTTAGAGAC | ||||||
PA83-1 | F: CCCTCTTTCTCTCATTGTCC | (TC)9 | 190–198 | 54 | 2 | 0.35 |
R: GGGACAGAGTGCATAAGATG | ||||||
PA83-2 | F: CCTTATCTCTTCTCTCTACC | (TC)36 | 168–210 | 50 | 12 | 0.87 |
R: AGAAAGGAAGGGTAGGAGAG | ||||||
PA100-1 | F: TCCCTCTATTTTAGACACCC | (TC)11 | 132–136 | 52 | 2 | 0.35 |
R: GGAGAAAGAGCAAGACAGTG | ||||||
PA100-2 | F: TCTCCATCCGTTAGCCTCTC | (CT)16 | 128–136 | 59 | 5 | 0.73 |
R: GGGTAGGCAGAGAGAGTGAT | ||||||
PA101 | F: CCCACTCACACTCTATCTTC | (TC)11 | 126–138 | 55 | 7 | 0.63 |
R: AGGGTCAAACAGAATGAAGG | ||||||
Average | 4.57 | 0.63 |