Fig. 4

LhSorNPR1 and LhSorTGA2 protein interaction assessed by the yeast two hybrid assay. a Yeast AH109 cells co-transformed with bait and prey vectors were grown on double dropout (DDO, SD/-Leu/-Trp) and quadruple dropout (QDO, SD/-Ade/-His/-Leu/-Trp) mediums. b The diagram indicates the corresponding co-transformants for the assay in c. c Transformants were streaked on QDO medium supplemented with 40 µg/ml X-α-Gal. pGBKT7-53 and pGADT7-T co-transformant was used as the positive control. Yeast cells co-transformed with pGBKT7 and pGADT7, pGBKT7-LhSorNPR1 and pGADT7, pGBKT7 and pGADT7-LhSorTGA2, or pGBKT7-Lam and pGADT7-T were used as negative controls