Fig. 4

Cytochemical localization of hydrogen peroxide (H₂O₂) in leaves of Glycine max cv. “Nam Dan” under infestation of Aphis craccivora and inoculation of Nostoc calcicola HN9 extract at 24 hpi. Green fluorescence came from DCFH-DA (dichlorodihydro-fluorescein diacetate), which is observed by a Zeiss LSM 510 confocal microscope, with excitation at 488 nm, emission at 500–550 nm, objective magnification of 63×, scale bar 10 µm