A method for facilitating the seed germination of a mycoheterotrophic orchid, Gastrodia pubilabiata, using decomposed leaf litter harboring a basidiomycete fungus, Mycena sp.

Botanical Studies

Table 1 The culture vessels and leaf litter used in this study and the results of seed germination

Vessel number	Size of the vessels	Location of leaf litter collected	Seed germination^b
Box 1	L260 × W190 × D100^a	Site 1	++
Box 2	L411 × W337 × D139	Site 1	+
Bottle 3	450 mL	Site 2	+
Bottle 4	450 mL	Site 2	−
Bottle 5	900 mL	Site 2	+

^aLength (L) × width (W) × depth (D) (mm)
^b++, a large number of protocorms and seedlings were observed; +, germination was observed; –, no germination