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Fig. 6 | Botanical Studies

Fig. 6

From: Actinidia callosa var. callosa suppresses metastatic potential of human hepatoma cell SK-Hep1 by inhibiting matrix metalloproteinase-2 through PI3K/Akt and MAPK signaling pathways

Fig. 6

Effects of betulinic acid, ursolic acid, and oleanolic acid on SK-Hep1 cell MMP-2 activity. The cells were incubated for 24 h in the absence or present of betulinic acid, ursolic acid and oleanolic acid (0, 3.125, 6.25, 12.5, 25, and 50 μM). After treatment, cell viability assay was performed using MTT assay. Optical density was determined at 570 nm and is expressed as cell survival relative to control. The conditioned media were collected, and MMP-2 activity was determined by gelatin zymography. MMP-2 activity was quantified by densitomeric analysis. The data were presented as mean ± SD for three different experiments performed in triplicate. *p < 0.05, **p < 0.01, and ***p < 0.001 was compared with untreated control group

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