Table 1 Sequences of PCR primers used in this study
Target code | Sequence (5′ → 3′) | Purpose |
|---|---|---|
P1-S | AATGACTTTGGAATGGGAGTTTG | Conserved fragment cloning |
P1-A | TAAATAGTTCAGGAGCCTGAGC | Conserved fragment cloning |
P2-S | GGCTTAGGAAAGTTTGATGCTG | 3′-RACE outer PCR |
P3-S | CTGTTACTGCTGGAAATGCCTCTA | 3′-RACE inner PCR |
P2-A | CCGCATCACCATATCCACGTATTCTAG | 5′-RACE outer PCR |
P3-A | GTGCAGATATTCCACGCTCAAAGC | 5′-RACE inner PCR |
P4-S | ATTCCCCTTTCCTTCAATCTCAG | Full-length cDNA cloning |
P4-A | TGTAACAGACAGAACAGGATGGC | Full-length cDNA cloning |
Actin-S | GGTAACATTGTGCTCAGTGGTGG | Reference gene |
Actin-A | AACGACCTTAATCTTCATGCTGC | Reference gene |
P5-S | GCAGGACGAGGAAAATCATC | Quantitative real-time PCR |
P5-A | CCAGCAGTAACAGAACCACC | Quantitative real-time PCR |