Fig. 1

Flower developmental stages (A) and their corresponding pollen germination rate (B) in Phalaenopsis Little Gem Stripes. A Flower developmental stages are defined as: (1) tight floral bud; (2) sepal half-open; (3) flower half-open; (4) prior to anthesis; (5) flower fully open; (6) 1 day after fully open. B In vitro pollen germination of P. Little Gem Stripes was evaluated by incubating in Brewbaker and Kwack medium for 7 days at 25 ± 1 °C and then by Alexander staining. Bars represent mean ± SE, n = 4. Pollen germination rank was represented by ranks with area of pollinia stained: (0) no-germination; (1) 1–20%; (2) 21–40%; (3) 41–60%; (4) 61–80%; (5) 81–100%