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Table 2 The amount of seeds in mature capsule after hand pollination of stored pollinia at four temperatures

From: Phalaenopsis pollinia storage at sub-zero temperature and its pollen viability assessment

  Storage weeks
0 4 8 16 24 32 40 48 56 64 72 80 88 96
RT +++a ++ ND ND ND ND ND ND ND ND ND ND ND ND
4 °C +++ +++ +++ +++ ++ ++ + ND ND ND ND ND
− 20 °C +++ +++ ++ +++ +++ +++ ++ + ++ + + + +
− 80 °C +++ +++ ++ ++ ++ ++ ++ + + + + + + +
  1. RT, room temperature
  2. aND, not determined (the capsules failed to develop for 7 days after pollination); seeds content per capsule: lacking (−), low (+), abundant (++), and very abundant (+++), respectively