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Table 1 Effects of cytokinins on direct somatic embryogenesis from leaf explants of Tolumnia Louise Elmore ‘Elsa’, when the explants were cultured in darkness for 75 days

From: Plant regeneration via direct somatic embryogenesis from leaf explants of Tolumnia Louise Elmore ‘Elsa’

Cytokinins Concentration (mg l−1) % of explants with somatic proembryos % of explants with somatic globular embryos % of browning
Hormone-free control 0 0 f 0 a 85.0 abc
2iP 0.3 0 f 0 a 90.0 abc
1 20.0 def 0 a 75.0 c
3 5.0 ef 0 a 80.0 bc
BA 0.3 0 f 0 a 30.0 d
1 35.0 abcd 0 a 20.0 de
3 15.0 def 0 a 10.0 de
Kinetin 0.3 0 f 0 a 100.0 a
1 0 f 0 a 80.0 bc
3 0 f 0 a 95.0 ab
TDZ 0.3 50.0 abc 5.0 a 5.0 de
1 45.0 abc 10.0 a 0 e
3 30.0 bcde 5.0 a 10.0 de
Zeatin 0.3 55.0 ab 0 a 10.0 de
1 65.0 a 0 a 10.0 de
3 30.0 cde 0 a 5.0 de
  1. Means within a column followed by the same letter are not significantly different according Duncan’s multiple range test at p ≤ 0.05 (Duncan 1955)