Table 2 Effects of cytokinins on direct somatic embryogenesis from leaf explants of Tolumnia Louise Elmore ‘Elsa’, when the explants were cultured in darkness for 90 days
Cytokinins | Concentration (mg l−1) | % of explants with somatic proembryos | % of explants with somatic globular embryos | % of browning |
|---|---|---|---|---|
Hormone-free control | 0 | 0 d | 0 d | 100.0 a |
2iP | 0.3 | 0 d | 0 d | 95.0 ab |
1.0 | 20.0 cd | 0 d | 85.0 ab | |
3.0 | 5.0 d | 0 d | 85.0 ab | |
BA | 0.3 | 5.0 d | 0 d | 70.0 b |
1.0 | 60.0 ab | 10.0 cd | 35.0 c | |
3.0 | 30.0 bc | 5.0 cd | 10.0 cd | |
Kinetin | 0.3 | 0 d | 0 d | 100.0 a |
1.0 | 0 d | 0 d | 90.0 ab | |
3.0 | 0 d | 0 d | 95.0 ab | |
TDZ | 0.3 | 42.5 ab | 15.0 bcd | 5.0 d |
1.0 | 75.0 a | 20.0 abc | 20.0 cd | |
3.0 | 90.0 a | 35.0 a | 15.0 cd | |
Zeatin | 0.3 | 70.0 a | 5.0 cd | 10.0 cd |
1.0 | 90.0 a | 20.0 abc | 10.0 cd | |
3.0 | 70.0 a | 30.0 ab | 10.0 cd |