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Table 2 Effects of cytokinins on direct somatic embryogenesis from leaf explants of Tolumnia Louise Elmore ‘Elsa’, when the explants were cultured in darkness for 90 days

From: Plant regeneration via direct somatic embryogenesis from leaf explants of Tolumnia Louise Elmore ‘Elsa’

Cytokinins Concentration (mg l−1) % of explants with somatic proembryos % of explants with somatic globular embryos % of browning
Hormone-free control 0 0 d 0 d 100.0 a
2iP 0.3 0 d 0 d 95.0 ab
1.0 20.0 cd 0 d 85.0 ab
3.0 5.0 d 0 d 85.0 ab
BA 0.3 5.0 d 0 d 70.0 b
1.0 60.0 ab 10.0 cd 35.0 c
3.0 30.0 bc 5.0 cd 10.0 cd
Kinetin 0.3 0 d 0 d 100.0 a
1.0 0 d 0 d 90.0 ab
3.0 0 d 0 d 95.0 ab
TDZ 0.3 42.5 ab 15.0 bcd 5.0 d
1.0 75.0 a 20.0 abc 20.0 cd
3.0 90.0 a 35.0 a 15.0 cd
Zeatin 0.3 70.0 a 5.0 cd 10.0 cd
1.0 90.0 a 20.0 abc 10.0 cd
3.0 70.0 a 30.0 ab 10.0 cd
  1. Means within a column followed by the same letter are not significantly different according Duncan’s multiple range test at p ≤ 0.05 (Duncan 1955)