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Table 3 Effects of 2,4-D plus TDZ on direct somatic embryogenesis from leaf explants of Tolumnia Louise Elmore ‘Elsa’, when the explants were cultured in darkness for 90 days

From: Plant regeneration via direct somatic embryogenesis from leaf explants of Tolumnia Louise Elmore ‘Elsa’

2,4-D (mg l−1)

TDZ (mg l−1)

% of explants with somatic proembryos

% of explants with somatic globular embryos

% of browning

0

0

0 a

0

100.0 a

1

0

0 a

0

100.0 a

1

0.3

5.0 a

0

100.0 a

1

3

10.0 a

0

100.0 a

  1. Means within a column followed by the same letter are not significantly different according Duncan’s multiple range test at p ≤ 0.05 (Duncan 1955)