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Botanical Studies

Table 4 Effects of TDZ and zeatin on direct somatic embryogenesis from leaf explants of Tolumnia Louise Elmore ‘Elsa’, when the explants were cultured in light condition for 90 days

From: Plant regeneration via direct somatic embryogenesis from leaf explants of Tolumnia Louise Elmore ‘Elsa’

Cytokinins

Concentration (mg l−1)

% of explants with somatic proembryos

% of explants with somatic globular embryos

% of browning

Hormone-free control

0

0 c

0

100.0 a

TDZ

1

10.0 abc

0

100.0 a

3

5.0 bc

0

100.0 a

Zeatin

1

25.0 ab

0

100.0 a

3

40.0 a

0

95.0 a

  1. Means within a column followed by the same letter are not significantly different according Duncan’s multiple range test at p ≤ 0.05 (Duncan 1955)
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