Table 1 Primer sequences used in real-time PCR analysis
Gene and abbreviation | Forward primer (5′–3′) | Reverse primer |
|---|---|---|
β-Actin | CTGAGGTCCTTTTCCAACCA | TACCCGGGAACATGGTAGAG |
CL10247 Glutamyl-tRNA reductase (Glu-TR) | TGGTGTCCGTTTCGCTGT | GCTGTGGTATCTTCTGGCTTTTC |
CL941. Contig 3 Ferri chelatase (FeCH) | GGACGAGAAATGAATCCTACCG | GCCCCGACAGAACAAAACA |
Gi2318136 Magnesium chelatase (MgCH) | TGATGCCACATTCCAGAACC | GGCTTCTTCCCGTCTTTCCT |
CL1870. Contig 2 Protochlorophyllide oxidoreductase (POR) | CCACGAAGAAACTGGCATTACA | CTGGAATGGAGGGAAAAGGAG |
CL4058. Contig 1 Chlorophyllase (CHLase) | CTCTCTTCCCAGCTTGTGCTC | CCTTGCCTCTAATCCCTTTTGTT |
Unigene40510_Rwg20A Mg-dechelatase (MDcase) | TCTATCCATCATCTGGCGTTTG | GGCTTCTTCCCGTCTTTCCT |
Unigene38644_Rwg20A Phaeophorbide a monooxygenase (PaO) | ATCGCTTCGTCTTGGCATTT | TCTATCCATCATCTGGCGTTTG |
CL4383.Contig 1 Phytoene synthase (PSY) | GGAATTTGGGCTTGTTGAGTG | TTGCGGAGTTATGTGGGATG |