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Table 1 The sequences of the primers used in qRT-PCR

From: Acetylsalicylic acid enhance tolerance of Phaseolus vulgaris L. to chilling stress, improving photosynthesis, antioxidants and expression of cold stress responsive genes

Primer name

 

Primer sequence (5_–3_)

Annealing temperature (°C)

CBF3

F

R

5′-TTCCGTCCGTACAGTGGAAT-3′

5′-AACTCCATAACGATACGTCGTC-3′

58

COR47

F

R

5′-CGGTACCAGTGTCGGAGAGT-3′

5′-ACAGCTGGTGAATCCTCTGC-3′

58

β-Actin

F

R

5′-CGCGACCTCACAGACTACCTG-3′

5′-CGTAGGACTTCTCCAGGGAGC-3′

58