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Table 2 Shoot fresh weight (g plant−1), dry weight (g plant−1), shoot: root ratio (g g−1), chlorophyll-a (mg g−1 FW), chlorophyll-b (mg g−1 FW), chlorophyll a/b, and total chlorophyll contents (mg g−1 FW) of P. vulgaris L. after foliar application of different concentration of acetylsalicylic acid (ASA; 0, 0.1, 0.5, 1, 2, 3 mM) and exposed to different level of chilling stress (0, 2, 4 days)

From: Acetylsalicylic acid enhance tolerance of Phaseolus vulgaris L. to chilling stress, improving photosynthesis, antioxidants and expression of cold stress responsive genes

Chilling (d) ASA foliar spray (mM) Shoot FW (g plant−1) Shoot DW (g plant−1) Shoot: root ratio (g g−1) Chl-a (mg g−1 FW) Chl-b (mg g−1 FW) Chl a/b Total chlorophyll (mg g−1 FW)
0 0 2.85 ± 0.002 g 1.65 ± 0.00ij 0.78 ± 0.09i 6.95 ± 0.01 h 3.75 ± 0.03de 1.85 ± 0.01i 10.70 ± 0.04a
0.1 2.87 ± 0.02 g 1.64 ± 0.00i 0.69 ± 0.01i 6.92 ± 0.00 h 3.74 ± 0.02de 1.85 ± 0.01i 10.67 ± 0.02a
0.5 2.85 ± 0.01 g 1.66 ± 0.02ij 0.68 ± 0.00i 6.95 ± 0.01 h 3.76 ± 0.02de 1.85 ± 0.00i 10.71 ± 0.03a
1 2.85 ± 0.01 g 1.66 ± 0.00ij 0.70 ± 0.01i 6.93 ± 0.00 h 3.71 ± 0.00def 1.87 ± 0.00i 10.64 ± 0.00a
2 2.85 ± 0.00 g 1.64 ± 0.02ij 0.68 ± 0.01i 6.96 ± 0.03 h 3.72 ± 0.00ef 1.87 ± 0.01i 10.68 ± 0.03a
3 2.85 ± 0.00 g 1.72 ± 0.05j 0.70 ± 0.01i 6.96 ± 0.02 h 3.72 ± 0.00ef 1.87 ± 0.01i 10.68 ± 0.02ab
2 0 1.67 ± 0.03bc 0.64 ± 0.01b 2.25 ± 0.05b 4.86 ± 0.00b 2.68 ± 0.00d 1.82 ± 0.00b 7.54 ± 0.00bc
0.1 1.81 ± 0.04bcd 0.68 ± 0.00bc 1.75 ± 0.35bc 4.92 ± 0.00c 2.97 ± 0.00b 1.66 ± 0.00c 7.88 ± 0.00c
0.5 1.92 ± 0.01d 0.86 ± 0.00d 1.85 ± 0.28ef 5.13 ± 0.00 fg 3.34 ± 0.00a 1.54 ± 0.00 fg 8.48 ± 0.00c
1 2.11 ± 0.08e 1.25 ± 0.01 g 1.39 ± 0.01f 5.22 ± 0.01de 3.18 ± 0.00b 1.64 ± 0.00ef 8.40 ± 0.01a
2 1.82 ± 0.03 cd 0.73 ± 0.01c 1.48 ± 0.00f 5.18 ± 0.00d 3.12 ± 0.10b 1.66 ± 0.05de 8.29 ± 0.10a
3 1.73 ± 0.05bc 0.83 ± 0.01d 1.86 ± 0.02de 5.07 ± 0.05c 2.95 ± 0.02c 1.72 ± 0.03c 8.02 ± 0.03a
4 0 0.94 ± 0.03a 0.55 ± 0.00a 2.56 ± 0.01a 4.75 ± 0.02a 2.37 ± 0.00 g 2.01 ± 0.01a 7.12 ± 0.01a
0.1 1.79 ± 0.01bcd 0.71 ± 0.00bc 1.69 ± 0.01 cd 4.98 ± 0.01def 3.22 ± 0.01a 1.55 ± 0.00d 8.21 ± 0.02a
0.5 2.17 ± 0.01e 1.16 ± 0.01f 1.59 ± 0.04 h 5.53 ± 0.01 g 3.41 ± 0.01b 1.62 ± 0.01 h 8.94 ± 0.00ab
1 2.48 ± 0.02f 1.72 ± 0.01j 0.88 ± 0.01 h 5.74 ± 0.02efg 3.28 ± 0.00c 1.75 ± 0.01 h 9.02 ± 0.01bc
2 2.25 ± 0.00e 1.44 ± 0.01 h 1.48 ± 0.01 h 5.70 ± 0.01c 2.86 ± 0.00 g 2.00 ± 0.00 fg 8.56 ± 0.01c
3 1.66 ± 0.01b 0.97 ± 0.02e 1.77 ± 0.06 h 5.64 ± 0.00c 2.95 ± 0.01f 1.91 ± 0.00 g 8.59 ± 0.00c
Univariate; two-way ANOVA
 F(corrected) 423.8*** 1013.3*** 2559.6*** 263.5*** 85.6*** 1768.3*** 34.23***
 F(intercept) 111,905*** 131,774*** 2,011,183*** 286,459*** 239,993*** 1,652,469*** 622.28***
 F(ASA) 2540.9*** 5482.6*** 20,123*** 1506.7*** 232.4*** 13,321.5*** 68.25***
 F(chilling) 203.1*** 686.9*** 353.3*** 169.7*** 104.2*** 395.9*** 4.87**
 F(ASA*chilling) 110.8*** 282.7*** 149.9*** 61.9*** 46.95*** 143.9*** 42.11***
  1. Data represented are mean of three points ± standard deviation
  2. * Significant at p < 0.05; ** p < 0.01; *** p value < 0.001, Variations between different chilling stresses and foliar ASA concentration were assessed by univariate analyses followed by Duncan’s test statistic. Means with the same letters are not significantly different according to Duncan’s multiple comparisons