Fig. 1

Generation of fluorescently labeled S. fimicola strains. a Schematic presentation of the plasmid construct used to generate histone H2B-mCherry-labeled strains. b PCR was used to verify the successful generation of the H2B-mCherry-labeled strains. The plasmid construct was ectopically integrated into the S. fimicola wild type strain (WT: BCRC33665). The transformants and wild-type strain are shown in Lanes 1–5 and WT, respectively. Amplification with primer pairs WC1218/WC2904 (left), WC2903/WC602 (middle), and ITS1/ITS4 (right) generated fragments of 974 bp, 1.1 kb, and 584 bp, respectively