Fig. 2

Live-cell imaging of nuclear distribution during the S. fimicola life cycle. The H2B-mCherry-labeled strain was cultured on malt extract agar III under constant white light. The nuclear distribution in a single germination vesicle and germ tube from an ascospore was observed at 2, 3, and 6 h post-inoculation (hpi). At 15 hpi, nuclear fluorescence was distributed in the mature vegetative hyphae and hyphal fusion regions, whereas the nuclei were not detected in the hyphal tip regions of the mature vegetative hyphae. Nuclear distribution in the coil and ascogonia hyphae, including the early protoperithecia, was detected within 27 and 48 hpi, respectively. At approximately 192 hpi, mature perithecia with asci containing black mature and multinucleated ascospores were observed. Live images were captured with the Deltavision core system. Nuclear distribution in each developmental stages of S. fimicola including germination vesical formation (a), hyphal extension and contact (b), ascogonial coils formation (c), differentiated ascogenous hyphae development (protoperithecia) (d) and perithecia formation (e) are showed. White arrows and arrowheads indicate hyphal fusion and the extension tip of a vegetative hypha, respectively. Yellow asterisks indicate vacuoles. Scale bars represent 12 μm