Fig. 3

Nuclear distribution of germ tube and mature vegetative hyphal tip and representative hyphal fusion events. The ascospores of the H2B-mCherry-labeled strain were grown on malt extract agar III under uniform constant white light. a Nuclear florescence was evenly distributed throughout the germ tube at 6 h post-inoculation (hpi). b At 15 hpi, the nuclei in the elongated vegetative hyphae (left) were absent in the tip regions, whereas nuclei were detected in the apical regions of pre-fusion hyphae [either tip-to-tip (middle) or tip-to-side (right)]. Live images were captured with the Deltavision core system. White arrows indicate differentiated sites of nuclear distribution among these fungal structures. White arrowheads indicate germination vesicles. Scale bars represent 12 μm