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Fig. 1 | Botanical Studies

Fig. 1

From: Grain width 2 (GW2) and its interacting proteins regulate seed development in rice (Oryza sativa L.)

Fig. 1

Proteomic analysis of seed proteins in the grain width 2 (gw2) mutant of rice, Oochikara. Total proteins were extracted from whole seeds and subjected to isoelectric focusing (IEF) in the first dimension, using immobilized pH gradient dry strips (pH 4–7 nonlinear, 24 cm in length), and SDS-PAGE in the second dimension, using 10–16% gradient polyacrylamide gels. Gels were stained with silver nitrate. Three independent biological replicates were performed. a 2D gels showing protein spots in wild-type and gw2 mutant rice. Arrowheads indicate highly accumulated proteins in the gw2 mutant compared with the wild type. Boxed regions shown in a are magnified in b. Spot #1, glyceraldehyde 3-phosphate dehydrogenase (GAPDH); spot #2, chitinase 14 (CHT14); spot #3, phosphoglycerate kinase (PGK)

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