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Fig. 2 | Botanical Studies

Fig. 2

From: Grain width 2 (GW2) and its interacting proteins regulate seed development in rice (Oryza sativa L.)

Fig. 2

GW2 interacts with PGK and CHT14. a Recombinant fusion proteins MBP-GW2, GST-PGK, and GST-CHT14 were overexpressed in E. coli and purified using amylose or glutathione resins. Arrowheads indicate MBP-GW2, GST-PGK, and GST-CHT14. b, c In vitro pull-down assay showing the proteins GST-PGK (b) and GST-CHT14 (c) pulled down with MBP-GW2, separated on 10% SDS–polyacrylamide gels, and detected by western blotting using anti-GST antibody. GST glutathione S-transferase, MBP maltose-binding protein, GW2 grain width 2, PGK phosphoglycerate kinase, CHT14 chitinase 14

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