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Fig. 3 | Botanical Studies

Fig. 3

From: Grain width 2 (GW2) and its interacting proteins regulate seed development in rice (Oryza sativa L.)

Fig. 3

GW2 does not ubiquitinate PGK and CHT14 in vitro. MBP-GW2, GST-PGK, and GST-CHT14 were overexpressed in E. coli and purified with amylose or glutathione affinity columns. Ubiquitination of GST-PGK (a) or GST-CHT14 (b) was examined in the presence or absence of rabbit E1, UbcH5b (E2), MBP-GW2 (E3), and His6-ubiquitin. PGK and CHT14 proteins were detected by western blotting using anti-GST antibody

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