Fig. 5
Gene-specific reverse transcriptase polymerase chain reaction (RT-PCR) analysis of CrCYC, CrRAD and CrDIV genes from C. ramondioides buds and dissected flower tissues. E, M, L represent three flower development stage defined in this study. E stands for early flower development stage; M stands for middle flower development stage; L stands for anthesis stage. P1 to P5 represent dissected petal from flower bud at early (E) flower developmental stage. Sep, Pe, Sta and gyn denote pooled sepals, pooled petals, pooled stamens and gynoecium dissected from early flower developmental stage. CrCYC1C, CrCYC1D and CrCYC2 indicate expression of CrCYC1C, CrCYC1D and CrCYC2. CrRAD1 and CrRAD2 indicate expression of CrRAD1 and CrRAD2. CrDIV indicates expression of CrDIV. 18S is included as a positive control. CrCYC1C, CrCYC1D, CrRAD1, CrRAD2 and CrDIV are detected through flower development stages, only CrCYC2 is restricted through flower development stages. CrDIV is detected in all petals, whereas CrCYC1C, CrCYC1D, CrCYC2, CrRAD1 and CrRAD2 are restricted in petals. CrCYC1C and CrCYC2 were detected in pooled sepal tissue