Fig. 7
From: Regulation of ABI5 expression by ABF3 during salt stress responses in Arabidopsis thaliana
The ABI5 promoter deletion assay. Schematic diagrams of the reporter, effector, and internal control plasmids used in the transient transactivation assay in Arabidopsis leaf protoplasts. a The reporter plasmid contained the ABI5 promoter. For the effector plasmid, the ABF3 gene was driven by the CaMV35S promoter. The PRL vector contained a CaMV35S promoter that drove the Renilla luciferase gene, Luc, as an internal control. b A schematic diagram of constructs of the ABI5 promoter with 5′ end deletions fused to the Luc reporter gene. D1 contains an ABRE cis-element (BOX 1 to BOX 5). D2 contains an ABRE cis-element (BOX 1 to BOX 4). D3 contains an ABRE cis-element (BOX 1 to BOX 3). D4 contains an ABRE cis-element (BOX 1). c The results indicated that through the deletion of the ABI5 promoter, transcriptional activity was decreased. ABF3 bound to BOX4 and BOX5 of the ABRE cis-element to regulate ABI5 transcription. Data are presented as the mean ± standard error (SE). N = 3, three biological repeats with three technical replicates for each biological repeat. The relative activity is calculated as the LUC firefly/LUC Renilla but is normalized to the minus ABF effector. The relative activity fold-change significantly differed from D1 is indicated by *P < 0.05 using the Student’s T-test