Fig. 8
From: Regulation of ABI5 expression by ABF3 during salt stress responses in Arabidopsis thaliana
ABF3 binds to the ABI5 promoter in vivo. The ChIP-qPCR analysis of the ABI5 promoter regions using the immunoprecipitation of the yellow fluorescent protein (YFP) in ABF3-overexpression lines using pEarleyGate 101 as the vector system. ABF3-YFP in Col is a transgenic line with YFP-tagged ABF3 expression in a Col background. Plants were grown on half-strength MS plates for 14 days followed with no treatment (a) or with 150 mM NaCl treatment (b) by 3 h. The enrichment of BOX 1 to BOX 5 was revealed by real-time PCR. UBQ10 and ACTIN2 were used as a negative control. Data are presented as the mean ± standard deviation (SD). N = 2, there were three technical replicates for each biological repeat (a total of n = 6). Significant differences between the vector only and ABF3 overexpression vectors are indicated by *P < 0.05 using the Student’s t-test