Comparative genomics of 11 complete chloroplast genomes of Senecioneae (Asteraceae) species: DNA barcodes and phylogenetics

Botanical Studies

Table 5 Details of ten primers that target the most divergent regions in the chloroplast genomes of Senecioneae species

Orientation	Primers	Tm	Target region	Expected product size
F	AAATAGGAGGAAGCTGTGAC	55	trnH-psbA	603
R	GCACTATGGCTTTCAACCTA	55
F	CACGTCAGATGTTCTATGGA	55	ndhD-ccsA	658
R	TTGGGCCTTTATTACTTGGA	55
F	TTTGCGAAAAGAGGAAGACA	55	trnT-trnL	812
R	TCCATACCAAGGCTCAATTC	55
F	TGTGGTAATTGCGTTGAGTA	55	ndhI-ndhG	759
R	TACGTAAATAAGGAGCTGCG	55
F	CTTGCTTGTACCTACCCAAT	55	trnS-rps4	588
R	TGACTCTTCAAGCATTCCAA	54
F	TTGACCTTGAAACAACAACG	55	rps18-rpl20	546
R	ACAAGAGACAGTTGCTTCTT	55
F	GGAACTAAAATGAACAGTGCA	54	psbM-trnD	880
R	ATGTCTACGCTGGTTCAAAT	55
F	GCGGATTCTAAATTGTAACCA	54	ndhC-trnV	959
R	AGCCCTAGAGCCCTATAAAA	55
F	TAACGCTGCCAAATATCCTT	55	rpl32-ndhF	1194
R	AGAAGAAGTCCCAACCCTAT	55
F	ATTGCCACTTCATCAATCTT	53	psbZ-trnG-	617
R	ACTACACTATGACGGCTAAC	54