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Fig. 3 | Botanical Studies

Fig. 3

From: Ethylene-mediated signaling confers thermotolerance and regulates transcript levels of heat shock factors in rice seedlings under heat stress

Fig. 3

ROS toxicity, cell death and antioxidant enzyme activity assay in rice seedlings under heat stress combined with ACC treatment. a DAB staining of H2O2 and evans blue staining of cell death in detached leaves of 12 days-old rice seedlings after treated 45 °C heat stress with or without 10 µM ACC for 4 days. Bar = 2 cm. Enzyme activity of catalase (b), ascorbate peroxidase (c), superoxide dismutase (d), and total peroxidase (e) in the shoots of 12-day-old rice seedlings was detected after 45 °C heat treatment with or without 10 µM ACC for 4 days. Control check (CK). Values are presented as mean ± standard deviation based on 30 seedlings of each treatment obtained from six biologically independent experiments. Different letters indicate significant difference between the treatments assessed using the LSD post hoc test (P < 0.05)

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