Fig. 4

Transcript levels of heat shock factor genes under heat stress with ACC treatment. Total RNA was isolated from the shoots of 12-day-old seedlings after heat stress (45 °C) with or without 10 µM ACC for 1 h. Relative expression levels of Os03g63750 (HsfA1a), Os03g53340 (HsfA2a), Os07g08140 (HsfA2b), Os10g28340 (HsfA2c), Os03g06630 (HsfA2d), Os03g58160 (HsfA2e), and Os06g36930 (HsfA2f) were determined through quantitative RT-PCR. Control check (CK). Values are presented as mean ± standard deviation based on 30 seedlings of each treatment obtained from six biologically independent experiments. Different letters indicate significant difference between the treatments assessed using the LSD post hoc test (P < 0.05)