Fig. 5

Transcript levels of ethylene biosynthesis related genes and ethylene signaling related genes under heat stress with ACC treatment. Total RNA was isolated from the shoots of 12-day-old seedlings after heat stress (45 °C) with or without 10 µM ACC for 1 h. Relative transcript levels were analyzed using quantitative RT-PCR. a Relative gene expression levels of Os01g39860 (ACC oxidase 1 [ACO1]) and Os05g05680 (ACC oxidase 3 [ACO3]) genes associated with ethylene biosynthesis pathways were determined using quantitative RT-PCR. b Relative gene expression levels of Os07g06130 (ethylene insensitive 2 [EIN2]), Os03g20780 (ethylene insensitive-like 1 [EIL1]), and Os07g48630 (ethylene insensitive-like 2 [EIL2]) genes associated with ethylene signaling pathways were determined using quantitative RT-PCR. Control check (CK). The data represent average values ± standard deviations based on 30 seedlings of each treatment obtained from six biologically independent experiments. Different letters indicate significant difference between the treatments assessed using the LSD post hoc test (P < 0.05)