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Table 1 Effects of autoclaving temperature and enzymatic digestion duration on the accuracy of starch measurement using this method

From: Optimization of a simple, accurate and low cost method for starch quantification in green microalgae

 Starch raw weight (μg)Starch net weight (μg)eStarch quantity measured by this method (μg)Accuracy (%)Mean ± SD
121 °C autoclave and single digestion15,45813,18612,03791.3 
 15,55513,26812,26692.5 
 15,22712,98911,76990.6 
 15,23712,99711,47988.390.7 ± 1.7a
(n = 4)
121 °C autoclave andouble digestions20,46217,45416,76296.0 
 24,67821,05019,54292.8 
 29,58225,23323,45593.0 
 32,89828,06225,86392.293.5 ± 1.7a,b
(n = 4)
134 °C autoclave and single digestionf20,59317,56616,13391.9 
 21,10718,00417,59697.7 
 26,98723,02021,99995.6 
 30,47625,99624,77995.3 
 35,67930,43428,45993.594.8 ± 2.2b,c
(n = 5)
134 °C autoclave and double digestionsf20,59317,55617,28298.4 
 21,10718,00417,76098.6 
 26,98723,02023,089100.3 
 30,47625,99625,46197.9 
 35,67930,43430,14999.198.9 ± 0.9d
(n = 5)
  1. The letters a, b, c and d after mean ± SD indicate significant difference (p < 0.05, t-test)
  2. eThe net weight excluded the weight of the water, protein and fiber in the corn starch
  3. fThe samples after autoclaving were split for single digestion and double digestions