Optimization of a simple, accurate and low cost method for starch quantification in green microalgae

Botanical Studies

Table 2 Accuracy of measuring the internal control cornstarch mixed with algal samples using this method

Net weight of internal control cornstarch (μg)^a	Algal starch quantified using this method (μg)	Combined starch in the mix of algal cells and internal control quantified using this method (μg)	Internal control determined using this method (μg)^b	Accuracy of internal control measurement (%)^c
0	4993
1240		6290	6290–4993 = 1297	104.6
0	4993
3917		9018	9018–4993 = 4025	102.8
0	4993
5522		10,802	10,802–4993 = 5809	105.2
0	5804
1051		6836	6836–5804 = 1032	98.2
0	5804
4033		9917	9917–5804 = 4113	102.0
0	5804
5748		11,613	11,613–5804 = 5809	101.1

^aThe net weight excluded the weight of the water, protein and fiber in the cornstarch
^bThe quantities were obtained by subtracting the amount of algal starch measured in the controls (without the cornstarch internal control) from the total starch
^cMean ± SD is 102.3 ± 2.5% (n = 6)