Fig. 3From: Functional characterization of NADPH-cytochrome P450 reductase and cinnamic acid 4-hydroxylase encoding genes from Scoparia dulcis L.HPLC analysis of 4-hydroxylase assays. E. coli cell lysates containing recombinant SdCPR and SdC4H (SdCPR/SdC4H) with NADPH (w/NADPH) or without NADPH (w/o NADPH). Empty vector (SdCPR/vector) with NADPH. Authentic standards of p-coumaric acid (Rt = 6.5 min) and t-cinnamic acid (Rt = 13.0 min) (Standard)Back to article page