Fig. 3

HPLC analysis of 4-hydroxylase assays. E. coli cell lysates containing recombinant SdCPR and SdC4H (SdCPR/SdC4H) with NADPH (w/NADPH) or without NADPH (w/o NADPH). Empty vector (SdCPR/vector) with NADPH. Authentic standards of p-coumaric acid (Rt = 6.5 min) and t-cinnamic acid (Rt = 13.0 min) (Standard)