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Fig. 4 | Botanical Studies

Fig. 4

From: Functional characterization of NADPH-cytochrome P450 reductase and cinnamic acid 4-hydroxylase encoding genes from Scoparia dulcis L.

Fig. 4

Real-time qPCR analysis of SdCPR and SdC4H genes. a Tissue-specific accumulation levels of the SdCPR and SdC4H genes in S. dulcis. Each organ was harvested from 8-week-old Scoparia plants for the isolation of total RNA. The transcript levels of each gene in young leaves were set to 1.0. b Effect of various treatments on expression levels of the SdCPR and SdC4H genes. The third leaves of Scoparia plant were treated with wounding, 0.1 mM methyl jasmonate (MJ) and 0.1 mM salicylic acid (SA). The transcript levels of each genes in the leaf at 0 h were set to 1.0. Each data was normalized to an internal control (GAPDH), and the ΔΔCT method was used to obtain relative values. Error bars represent the ± SD of the mean (n = 3)

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