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Table 3 Steady-state kinetic constant of cytochrome c and NADPH

From: Functional characterization of NADPH-cytochrome P450 reductase and cinnamic acid 4-hydroxylase encoding genes from Scoparia dulcis L.

  NADPH Cytochrome c
Vmax (μmol/min/mg−1) 2.3 ± 0.1 2.5 ± 0.1
Km (μM) 4.6 ± 0.9 2.7 ± 0.6
kcat (min−1) 177.6 ± 7.8 198.1 ± 6.6
kcat K −1m 39.9 ± 6.4 77.2 ± 16.4
  1. Steady-state kinetic constants of recombinant SdCPR at 28 ℃, pH 7.5. Determination of kinetic parameters for cytochrome c was performed in reaction mixture containing 100 μM NADPH and various amounts of cytochrome c, and kinetic parameters for NADPH was determined by using 100 μM cytochrome c as substrate and various amounts of NADPH (n = 3). Value are presented as mean ± SE