| NADPH | Cytochrome c |
---|
Vmax (μmol/min/mg−1) | 2.3 ± 0.1 | 2.5 ± 0.1 |
Km (μM) | 4.6 ± 0.9 | 2.7 ± 0.6 |
kcat (min−1) | 177.6 ± 7.8 | 198.1 ± 6.6 |
kcat K
−1m
| 39.9 ± 6.4 | 77.2 ± 16.4 |
- Steady-state kinetic constants of recombinant SdCPR at 28 ℃, pH 7.5. Determination of kinetic parameters for cytochrome c was performed in reaction mixture containing 100 μM NADPH and various amounts of cytochrome c, and kinetic parameters for NADPH was determined by using 100 μM cytochrome c as substrate and various amounts of NADPH (n = 3). Value are presented as mean ± SE