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Table 4 Effects of different sections/incisions and solid and liquid medium on bulblet regeneration in F. cirrhosa

From: In vitro propagation of bulblets and LC–MS/MS analysis of isosteroidal alkaloids in tissue culture derived materials of Chinese medicinal herb Fritillaria cirrhosa D. Don

Medium1Treatment2Explant survival (%)3Explants with bulblet formation (%)3No. of bulblet produced3
LiquidA25.0c91.7a16.4bc
B41.7bc91.7a7.0bc
C75.0bc91.7a3.9c
D58.3abc91.7a5.3bc
SolidA66.7ab100.0a11.5b
B66.7ab100.0a26.7a
C91.7a100.0a8.6bc
D83.3a100.0a7.2bc
  1. 11/2 X MSBM with 2.5% sucrose and 0.4% GPP. Cultures were incubated at 20 °C. Data were recorded after 2 months of culture. Each treatment had 12 replicates
  2. 2A: longitudinal sections; B transverse sections; C: longitudinal incision; D: transverse incision
  3. 3Means followed by the different letter of a column are significantly different at 5% level by LSD test