In vitro propagation of bulblets and LC–MS/MS analysis of isosteroidal alkaloids in tissue culture derived materials of Chinese medicinal herb Fritillaria cirrhosa D. Don

Botanical Studies

Table 4 Effects of different sections/incisions and solid and liquid medium on bulblet regeneration in F. cirrhosa

Medium¹	Treatment²	Explant survival (%)³	Explants with bulblet formation (%)³	No. of bulblet produced³
Liquid	A	25.0^c	91.7^a1	6.4^bc
	B	41.7^bc	91.7^a	7.0^bc
	C	75.0^bc	91.7^a	3.9^c
	D	58.3^abc	91.7^a	5.3^bc
Solid	A	66.7^ab	100.0^a	11.5^b
	B	66.7^ab	100.0^a	26.7^a
	C	91.7^a	100.0^a	8.6^bc
	D	83.3^a	100.0^a	7.2^bc

¹1/2 X MSBM with 2.5% sucrose and 0.4% GPP. Cultures were incubated at 20 °C. Data were recorded after 2 months of culture. Each treatment had 12 replicates
²A: longitudinal sections; B transverse sections; C: longitudinal incision; D: transverse incision
³Means followed by the different letter of a column are significantly different at 5% level by LSD test